shinya yamanaka (Addgene inc)
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shinya yamanaka
Shinya Yamanaka, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shinya+yamanaka/pMXs-DsRed+Express+(Plasmid+%2322724)/pm41398047-51-39-41
Average 93 stars, based on 17 article reviews
Shinya Yamanaka, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shinya+yamanaka/pMXs-DsRed+Express+(Plasmid+%2322724)/pm41398047-51-39-41
Average 93 stars, based on 17 article reviews
shinya yamanaka - by Bioz Stars,
2026-10
93/100 stars
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Transfection:Article Title: MSH2 is not required for either maintenance of DNA methylation or repeat contraction at the FMR1 locus in fragile X syndrome or the FXN locus in Friedreich's ataxia. Article Snippet: .. Cells were transfected with pX462-MSH2-dualgRNA and pCE-mp53DD, a gift from Plasmid Preparation:Article Title: MSH2 is not required for either maintenance of DNA methylation or repeat contraction at the FMR1 locus in fragile X syndrome or the FXN locus in Friedreich's ataxia. Article Snippet: .. Cells were transfected with pX462-MSH2-dualgRNA and pCE-mp53DD, a gift from Article Title: Generation of four iPSC lines from a family harboring a 1p36-35 haplotype linked with bipolar disorder and recurrent depressive disorder: Three-generation patients and a healthy sibling. Article Snippet: .. 1383D6 (HPS1006) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan. pCXLE-hoct3/4-shp53-F, pCXLE-hSK, pCXLE-hUL, and pCXWBEBNA1 were gifts from Article Title: SLAP controls mTORC2 integrity via UBE3C-mediated non-degradative mLST8 ubiquitination to suppress colorectal tumorigenesis. Article Snippet: Drugs: mTOR inhibitors KU-0063794, Temsilorimus (TS), and AZD2014 were from Selleckchem (USA); PP242/Torkinib (#T2414) was from TargetMOL. .. Vectors: pcDNA3 and retroviral pMX-pS-CESAR vectors expressing SLAPFlag, SLAP SH3*(P73L)-Flag, SLAP SH2*(R111E)-Flag, and SLAP SH3*SH2*Flag (a gift of A. Weiss, University of California, San Francisco, USA) and SLAP N32-Flag were described in [29]. pMXs-DsRed Express was a gift from Article Title: Gene electrotransfer with flow-through microchannel and lower alternating voltage generated induced pluripotent cells from human lymphoblastoid cell lines Article Snippet: .. The episomal plasmid vector pCXLE-EGFP, used to express GFP, was gifted by Article Title: Generation of a FAM189A2/ENTREP1 knockout human induced pluripotent stem cell line using CRISPR/Cas9 technology Article Snippet: .. The cell culture system used Feeder free medium / mTeSR1TM Type of the Genetic Modification Induced deletions by CRISP Cas9 in ENTREP1 gene Associated disease Cancer, cardiovascular diseases Gene/locus modified in the reported transgenic line ENTREP1 gene.9q21.2 NM_001347995.2:c.554_555del and NM_001347995.2:c.553_572del lead to premature stop codons, NP_001334924.1:p.Phe185CysfsTer8 and NP_001334924.1:p.Phe185ValfsTer2 Method of modification / user-customisable nucleases (UCN) used, the resource used for design optimisation CRISPR/Cas9 induced non-homologous end-joining (NHEJ) User-customisable nuclease (UCN) delivery method Electroporation with an Amaxa 4D-Nucleofector (Lonza) All double-stranded DNA genetic material molecules introduced into the cells pSpCas9(BB)-2A-GFP (pX458) was a gift from Feng Zhang (Addgene plasmid # 48,138; https://n2t.net/addgene:48138; RRID:Addgene_48138) pCX-cMyc was a gift from Modification:Article Title: Null and missense mutations of ERI1 cause a recessive phenotypic dichotomy in humans. Article Snippet: .. Note The Americ ming of LCLs was performed with episomes as previously described with a minor modification.27 Briefly, LCLs were electroporated with four episomal vectors (pCXLE-hOCT3/4-shp53-F, pCXLE-hSK, pCXLE-hUL, and pCXWB-EBNA1), which were gifts from Article Title: Generation of a FAM189A2/ENTREP1 knockout human induced pluripotent stem cell line using CRISPR/Cas9 technology Article Snippet: .. The cell culture system used Feeder free medium / mTeSR1TM Type of the Genetic Modification Induced deletions by CRISP Cas9 in ENTREP1 gene Associated disease Cancer, cardiovascular diseases Gene/locus modified in the reported transgenic line ENTREP1 gene.9q21.2 NM_001347995.2:c.554_555del and NM_001347995.2:c.553_572del lead to premature stop codons, NP_001334924.1:p.Phe185CysfsTer8 and NP_001334924.1:p.Phe185ValfsTer2 Method of modification / user-customisable nucleases (UCN) used, the resource used for design optimisation CRISPR/Cas9 induced non-homologous end-joining (NHEJ) User-customisable nuclease (UCN) delivery method Electroporation with an Amaxa 4D-Nucleofector (Lonza) All double-stranded DNA genetic material molecules introduced into the cells pSpCas9(BB)-2A-GFP (pX458) was a gift from Feng Zhang (Addgene plasmid # 48,138; https://n2t.net/addgene:48138; RRID:Addgene_48138) pCX-cMyc was a gift from Retroviral:Article Title: SLAP controls mTORC2 integrity via UBE3C-mediated non-degradative mLST8 ubiquitination to suppress colorectal tumorigenesis. Article Snippet: Drugs: mTOR inhibitors KU-0063794, Temsilorimus (TS), and AZD2014 were from Selleckchem (USA); PP242/Torkinib (#T2414) was from TargetMOL. .. Vectors: pcDNA3 and retroviral pMX-pS-CESAR vectors expressing SLAPFlag, SLAP SH3*(P73L)-Flag, SLAP SH2*(R111E)-Flag, and SLAP SH3*SH2*Flag (a gift of A. Weiss, University of California, San Francisco, USA) and SLAP N32-Flag were described in [29]. pMXs-DsRed Express was a gift from Expressing:Article Title: SLAP controls mTORC2 integrity via UBE3C-mediated non-degradative mLST8 ubiquitination to suppress colorectal tumorigenesis. Article Snippet: Drugs: mTOR inhibitors KU-0063794, Temsilorimus (TS), and AZD2014 were from Selleckchem (USA); PP242/Torkinib (#T2414) was from TargetMOL. .. Vectors: pcDNA3 and retroviral pMX-pS-CESAR vectors expressing SLAPFlag, SLAP SH3*(P73L)-Flag, SLAP SH2*(R111E)-Flag, and SLAP SH3*SH2*Flag (a gift of A. Weiss, University of California, San Francisco, USA) and SLAP N32-Flag were described in [29]. pMXs-DsRed Express was a gift from Cell Culture:Article Title: Generation of a FAM189A2/ENTREP1 knockout human induced pluripotent stem cell line using CRISPR/Cas9 technology Article Snippet: .. The cell culture system used Feeder free medium / mTeSR1TM Type of the Genetic Modification Induced deletions by CRISP Cas9 in ENTREP1 gene Associated disease Cancer, cardiovascular diseases Gene/locus modified in the reported transgenic line ENTREP1 gene.9q21.2 NM_001347995.2:c.554_555del and NM_001347995.2:c.553_572del lead to premature stop codons, NP_001334924.1:p.Phe185CysfsTer8 and NP_001334924.1:p.Phe185ValfsTer2 Method of modification / user-customisable nucleases (UCN) used, the resource used for design optimisation CRISPR/Cas9 induced non-homologous end-joining (NHEJ) User-customisable nuclease (UCN) delivery method Electroporation with an Amaxa 4D-Nucleofector (Lonza) All double-stranded DNA genetic material molecules introduced into the cells pSpCas9(BB)-2A-GFP (pX458) was a gift from Feng Zhang (Addgene plasmid # 48,138; https://n2t.net/addgene:48138; RRID:Addgene_48138) pCX-cMyc was a gift from Transgenic Assay:Article Title: Generation of a FAM189A2/ENTREP1 knockout human induced pluripotent stem cell line using CRISPR/Cas9 technology Article Snippet: .. The cell culture system used Feeder free medium / mTeSR1TM Type of the Genetic Modification Induced deletions by CRISP Cas9 in ENTREP1 gene Associated disease Cancer, cardiovascular diseases Gene/locus modified in the reported transgenic line ENTREP1 gene.9q21.2 NM_001347995.2:c.554_555del and NM_001347995.2:c.553_572del lead to premature stop codons, NP_001334924.1:p.Phe185CysfsTer8 and NP_001334924.1:p.Phe185ValfsTer2 Method of modification / user-customisable nucleases (UCN) used, the resource used for design optimisation CRISPR/Cas9 induced non-homologous end-joining (NHEJ) User-customisable nuclease (UCN) delivery method Electroporation with an Amaxa 4D-Nucleofector (Lonza) All double-stranded DNA genetic material molecules introduced into the cells pSpCas9(BB)-2A-GFP (pX458) was a gift from Feng Zhang (Addgene plasmid # 48,138; https://n2t.net/addgene:48138; RRID:Addgene_48138) pCX-cMyc was a gift from CRISPR:Article Title: Generation of a FAM189A2/ENTREP1 knockout human induced pluripotent stem cell line using CRISPR/Cas9 technology Article Snippet: .. The cell culture system used Feeder free medium / mTeSR1TM Type of the Genetic Modification Induced deletions by CRISP Cas9 in ENTREP1 gene Associated disease Cancer, cardiovascular diseases Gene/locus modified in the reported transgenic line ENTREP1 gene.9q21.2 NM_001347995.2:c.554_555del and NM_001347995.2:c.553_572del lead to premature stop codons, NP_001334924.1:p.Phe185CysfsTer8 and NP_001334924.1:p.Phe185ValfsTer2 Method of modification / user-customisable nucleases (UCN) used, the resource used for design optimisation CRISPR/Cas9 induced non-homologous end-joining (NHEJ) User-customisable nuclease (UCN) delivery method Electroporation with an Amaxa 4D-Nucleofector (Lonza) All double-stranded DNA genetic material molecules introduced into the cells pSpCas9(BB)-2A-GFP (pX458) was a gift from Feng Zhang (Addgene plasmid # 48,138; https://n2t.net/addgene:48138; RRID:Addgene_48138) pCX-cMyc was a gift from Non-Homologous End Joining:Article Title: Generation of a FAM189A2/ENTREP1 knockout human induced pluripotent stem cell line using CRISPR/Cas9 technology Article Snippet: .. The cell culture system used Feeder free medium / mTeSR1TM Type of the Genetic Modification Induced deletions by CRISP Cas9 in ENTREP1 gene Associated disease Cancer, cardiovascular diseases Gene/locus modified in the reported transgenic line ENTREP1 gene.9q21.2 NM_001347995.2:c.554_555del and NM_001347995.2:c.553_572del lead to premature stop codons, NP_001334924.1:p.Phe185CysfsTer8 and NP_001334924.1:p.Phe185ValfsTer2 Method of modification / user-customisable nucleases (UCN) used, the resource used for design optimisation CRISPR/Cas9 induced non-homologous end-joining (NHEJ) User-customisable nuclease (UCN) delivery method Electroporation with an Amaxa 4D-Nucleofector (Lonza) All double-stranded DNA genetic material molecules introduced into the cells pSpCas9(BB)-2A-GFP (pX458) was a gift from Feng Zhang (Addgene plasmid # 48,138; https://n2t.net/addgene:48138; RRID:Addgene_48138) pCX-cMyc was a gift from Electroporation:Article Title: Generation of a FAM189A2/ENTREP1 knockout human induced pluripotent stem cell line using CRISPR/Cas9 technology Article Snippet: .. The cell culture system used Feeder free medium / mTeSR1TM Type of the Genetic Modification Induced deletions by CRISP Cas9 in ENTREP1 gene Associated disease Cancer, cardiovascular diseases Gene/locus modified in the reported transgenic line ENTREP1 gene.9q21.2 NM_001347995.2:c.554_555del and NM_001347995.2:c.553_572del lead to premature stop codons, NP_001334924.1:p.Phe185CysfsTer8 and NP_001334924.1:p.Phe185ValfsTer2 Method of modification / user-customisable nucleases (UCN) used, the resource used for design optimisation CRISPR/Cas9 induced non-homologous end-joining (NHEJ) User-customisable nuclease (UCN) delivery method Electroporation with an Amaxa 4D-Nucleofector (Lonza) All double-stranded DNA genetic material molecules introduced into the cells pSpCas9(BB)-2A-GFP (pX458) was a gift from Feng Zhang (Addgene plasmid # 48,138; https://n2t.net/addgene:48138; RRID:Addgene_48138) pCX-cMyc was a gift from |